Journal
ENZYME AND MICROBIAL TECHNOLOGY
Volume 26, Issue 8, Pages 614-620Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/S0141-0229(00)00136-8
Keywords
beta-1,3-glucosidase; cellobiose; Aspergillus niger; enzymatic hydrolysis
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We have made experimental studies into the enzymatic hydrolysis of cellobiose within the temperature range of 40 degrees C to 70 degrees C at pH 4.9, by using beta-1,4-glucosidase from Aspergillus niger. At 70 degrees C there was significant enzyme deactivation, which could be fitted to a potential deactivation model with values of n equal to 1.09 and k(d) to 0.1564 (g/l)(-0.09) min(-1), whereas the rate of hydrolysis could be fitted to the Michaelis-Menten equation. Between 40 degrees C and 60 degrees C we noted a substrate inhibition and that the CEC compound formed contributed to glucose production. The apparent activation energies had values of 4.66, 8.45, 4.82, and 3.99 kJ/mol for the kinetic constants k(a) and k(a2) the Michaelis constant and the substrate inhibition constant, respectively. (C) 2000 Elsevier Science Inc. All rights reserved.
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