Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 275, Issue 18, Pages 13974-13985Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.275.18.13974
Keywords
-
Categories
Funding
- NIDA NIH HHS [F31 DA05694] Funding Source: Medline
- NINDS NIH HHS [NS 13546] Funding Source: Medline
Ask authors/readers for more resources
Transient transfection has not been a successful method to express the alpha 7 nicotinic acetylcholine receptor such that these receptors are detected on the cell surface. This is not the case for all ligand-gated ion channels. Transient transfection with the 5-hydroxytryptamine type 3 subunit cDNA results in detectable surface receptor expression, Cell lines stably ex pressing the alpha 7 nicotinic acetylcholine receptor produce detectable, albeit variable, levels of surface receptor expression. alpha 7 nicotinic acetylcholine receptor surface expression is dependent, at least in part, on cell-specific factors, In addition to factors provided by the cells used for receptor expression, we hypothesize that the surface expression level in transfected cells is an intrinsic property of the receptor protein under study, Employing a set of alpha 7-5-hydroxytryptamine type 3 chimeric receptor subunit cDNAs, we expressed these constructs in a transient transfection system and quantified surface receptor expression. We have identified amino acids that control receptor distribution between surface and intracellular pools; surface receptor expression can be manipulated without affecting the total number of receptors, These determinants function independently of the cell line used for expression and the transfection method employed. How these surface expression determinants in the alpha 7 nicotinic acetylcholine receptor might influence synaptic efficacy is discussed.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available