Journal
FEMS MICROBIOLOGY LETTERS
Volume 186, Issue 2, Pages 235-238Publisher
ELSEVIER SCIENCE BV
DOI: 10.1111/j.1574-6968.2000.tb09110.x
Keywords
thermophilic lipase; cloning; overexpression; Bacillus thermoleovorans
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A thermophilic lipase of Bacillus thermoleovorans ID-1 was cloned and sequenced. The lipase gene codes 416 amino acid residues and contains the conserved pentapeptide Ala-X-Ser-X-Gly as other Bacillus lipase genes. The optimum temperature of the lipase is 75 degrees C. which is higher than other known Bacillus lipases. For expression in Escherichia coli, the lipase gene was subcloned in pET-22b(+) vector with a strong T7 promoter. Lipase activity was approximately 1.4-fold greater than under the native promoter. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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