Journal
JOURNAL OF CHROMATOGRAPHY B
Volume 742, Issue 1, Pages 99-108Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0378-4347(00)00141-9
Keywords
S-nitroso compounds; thiols
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Low-molecular-mass S-nitroso compounds (R-S-N=O) are potent vasodilators and inhibitors of platelet aggregation. This work describes the electrospray ionization mass spectrometric (ESI-MS) analysis of physiological and synthetic low-molecular-mass S-nitroso compounds and their thiols including S-nitrosoglutathione, S-nitrosocysteine, glutathione and cysteine. Mass spectra of the unlabeled and S-'5N-labeled low-molecular-mass S-nitroso compounds investigated are characterized by abundant cations due to [M+H](+), [M+Na](+), [(M+H)-NO](+), [2 M+H](+), and [(2 M+H)-2NO](+). Mass spectra of low-molecular-mass thiols are characterized by abundant cations due to [M+H](+), [M+Na](+) and [2M+H](+). Using off-line electrospray ionization tandem mass spectrometry we unequivocally identified S-[N-15]nitrosoglutathione in human red blood cells formed after their incubation with S-[N-15]nitrosocysteine. These results suggest that ESI-MS in combination with an appropriate liquid chromatographic system should be a useful analytical approach for the on-line quantitative determination of low-molecular-mass S-nitroso compounds in biological fluids in the presence of their thiols and nitrite. Considerations were made about on-line ESI-MS and quantitative measurements. (C) 2000 Elsevier Science B.V. All rights reserved.
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