Journal
FEBS LETTERS
Volume 474, Issue 1, Pages 11-15Publisher
WILEY
DOI: 10.1016/S0014-5793(00)01561-1
Keywords
poly(ADP-ribose) polymerase; apoptosis; caspase-3-like protease; 3-aminobenzamide; nicotinamide; tobacco
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The cleavage of poly(ADP-ribose) polymerase (PARP) by caspase (casp)-3 is an essential link in the apoptotic pathway in animal cells. In plant cells, however, there is no authentic evidence for the similar role that PARP may play during apoptosis, Using a heat shock (HS)-induced apoptosis system of tobacco cells, we found that immediately after a 4 h heat treatment, PARP was cleaved to form an 89 kDa signature fragment, while DNA laddering appeared only after a 20 h recovery following the HS, An activation of casp-3-like protease was also observed, The results suggest that apoptosis in plants and animals may share common mechanisms. On the other hand, when cells were preincubated with 4 mM 3-aminobenzamide or 2-8 mM nicotinamide, the specific inhibitors of PARP, before HS treatment, apoptotic cell death was reduced significantly. Our results thus imply that PARP may also be involved in apoptosis in a different way from the case-related events. (C) 2000 Federation of European Biochemical Societies.
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