Journal
FEBS LETTERS
Volume 474, Issue 1, Pages 16-22Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(00)01564-7
Keywords
motor protein; myosin; Rho-type GTPase; GDP/GTP exchange factor; MyoM; Dictyostelium discoideum
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We have cloned a novel unconventional myosin gene myoM in Dictyostelium, Phylogenetic analysis of the motor domain indicated that MyoM does not belong to any known subclass of the myosin superfamily, Following the motor domain, two calmodulin-binding IQ motifs, a putative coiled-coil region, and a Pro, Ser and Thr-rich domain, lies a combination of dbl homology and pleckstrin homology domains, These are conserved in Rho GDP/GTP exchange factors (RhoGEFs), We have identified for the first time the RhoGEF domain in the myosin sequences. The growth and terminal developmental phenotype of Dictyostelium cells were not affected by the myoM- mutation. Green fluorescent protein-tagged MyoM, however, accumulated at cro,vn-shaped projections and membranes of phase lucent vesicles in growing cells, suggesting its possible roles in macropinocytosis. (C) 2000 Federation of European Biochemical Societies.
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