Journal
EMBO JOURNAL
Volume 19, Issue 11, Pages 2465-2474Publisher
WILEY
DOI: 10.1093/emboj/19.11.2465
Keywords
AGM; AML; embryo; Runx1; vitelline
Categories
Funding
- FIC NIH HHS [FO6TW03300] Funding Source: Medline
- NCI NIH HHS [R0CA58343, R01 CA058343] Funding Source: Medline
- NIDDK NIH HHS [R01 DK51077-01] Funding Source: Medline
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The aorta-gonad-mesonephros (AGM) region is a potent hematopoietic site within the mammalian embryo body, and the first place from which hematopoietic stem cells (HSCs) emerge. Within the complex embryonic vascular, excretory and reproductive tissues of the AGM region, the precise location of HSC development is unknown. To determine where HSCs develop, we subdissected the AGM into aorta and urogenital ridge segments and transplanted the cells into irradiated adult recipients. We demonstrate that HSCs first appear in the dorsal aorta area. Furthermore, we show that vitelline and umbilical arteries contain high frequencies of HSCs coincident with HSC appearance in the AGM, While later in development and after organ explant culture we find HSCs in the urogenital ridges, our results strongly suggest that the major arteries of the embryo are the most important sites from which definitive HSCs first emerge.
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