Journal
SYSTEMATIC AND APPLIED MICROBIOLOGY
Volume 23, Issue 2, Pages 210-218Publisher
GUSTAV FISCHER VERLAG
DOI: 10.1016/S0723-2020(00)80007-7
Keywords
Methylopila helvetica; Methylobacterium dichloromethanicum taxonomy; phylogeny; facultative methylotrophs; dichloromethane; serine pathway
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Eight strains of Gram-negative, aerobic, asporogenous, neutrophilic, mesophilic, facultatively methylotrophic bacteria are taxonomically described. These icl(-) serine pathway methylobacteria utilize dichloromethane, methanol and methylamine as well as a variety of polycarbon compounds as the carbon and energy source. The major cellular fatty acids of the non-pigmented strains DM1, DM3, and DM5 to DM9 are C-18:1, C-16:0, C-18:0, C-cy19:0 and that of the pink-pigmented strain DM4 is C-18:1. The main quinone of all the strains is Q-10. The non-pigmented strains have similar phenotypic properties and a high level of DNA-DNA relatedness (81-98%) as determined by hybridization. All strains belong to the alpha-subgroup of the a-Proteobacteria. 16S rDNA sequence analysis led to the classification of these dichloromethane-utilizers in the genus Methylopila as a new species - Methylopila helvetica sp.nov. with the type strain DM9 (=VKM B-2189). The pink-pigmented strain DM4 belongs to the genus Methylobacterium but differs From the known members of this genus by some phenotypic properties, DNA-DNA relatedness (14-57%) and 16S rDNA sequence. Strain DM4 is named Methylobacterium dichloromethanicum sp. nov. (VKM B-2191 = DSMZ 6343).
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