Journal
MOLECULAR PLANT-MICROBE INTERACTIONS
Volume 13, Issue 6, Pages 583-591Publisher
AMER PHYTOPATHOLOGICAL SOC
DOI: 10.1094/MPMI.2000.13.6.583
Keywords
-
Ask authors/readers for more resources
The life genes (nfeA, nfeB, and nfeD) are involved in the nodulation efficiency and competitiveness of the Sinorhizobium meliloti strain GR4 on alfalfa roots. The nfeA and nfeB genes are preceded by functional nif consensus sequences and NifA binding motifs, Here, we determined the temporal and spatial expression patterns of the nfe genes in symbiosis with alfalfa, Translational fusions of the nfe promoters with the gusA gene and reverse transcription-polymerase chain reaction analyses indicate that they are expressed and translated within mature nitrogen-fixing modules and not during early steps of nodule development. Within the nodules the three nfe genes exhibit a spatial expression pattern similar to that of genes involved in symbiotic nitrogen fixation. We show that nfeB and nfeD genes are expressed not only from their own promoters but also from the upstream nfe promoter sequences. Furthermore, with the use of specific antibodies the NfeB and NfeD proteins were detected within the root nodule bacteroid fraction. Finally, NfeB was immunolocalized in the bacteroid cell membrane whereas NfeD was detected in the bacteroid cytoplasm.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available