Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 87, Issue 1-2, Pages 71-80Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0166-0934(00)00149-X
Keywords
HSV-2 antibody; Africa; ELISA
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A competitive type-specific enzyme-linked immunosorbent assay (ELISA) for herpes simplex virus type 2 (HSV-2) antibody was developed using an infected cell antigen and a monoclonal antibody to glycoprotein G-2. This assay has been validated for use for epidemiological studies using a large panel of sera collected in rural Uganda and a panel of 143 sera characterised previously by Western blotting, the 'gold standard' for HSV type-specific serology. This evaluation was found to have a sensitivity of 96% and a specificity of 91% in comparison with Western blot on 143 sera from clinic patients. The ELISA had a sensitivity of 93% and a specificity of 91% in comparison with Western blot on 495 sera collected in Uganda. The assay showed good reproducibility and a low percentage of sera gave equivocal results, indicating its suitability for epidemiological studies. (C) 2000 Published by Elsevier Science B.V. All rights reserved.
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