Expression and activity of protein kinase D/protein kinase Cμ in myocardium:: Evidence for α1-adrenergic receptor- and protein kinase C-mediated regulation

Protein kinase D (PKD), which is also known as protein kinase C (PKC) mu is a novel serine/threonine kinase that can be activated in parallel with or downstream of PKC in various cell types. but its expression and regulation in myocardium have not been characterized. In the present study, two proteins of 110 and 115 kDa were detected in rat ventricular myocardium using antibodies directed at the extreme N- or C-terminus of PKD. Both proteins were highly expressed in the fetal heart but showed a developmental decline in abundance. Fractionation studies showed that PKD was distributed between myocyte and non-myocyte fractions in the neonatal heart, but was found predominantly in the non-myocyte fraction in the adult heart. In cultured neonatal rat ventricular myocytes, an in vitro kinase assay revealed increased autophosphorylation of PKD (EC50 2.8 nM) in response to phorbol-12-myristate-13-acetate (PMA). Exposure to norepinephrine also induced a dose-dependent increase in PKD autophosphorylation (EC50 0.6 mu M). Pretreatment with the alpha(1)-adrenergic receptor (AR) antagonist prazosin blocked norepinephrine-induced PKD autophosphorylation, while the beta(1)-AR antagonist atenolol had no effect, indicating that activation of PKD by norepinephrine occurred via the alpha(1)-AR. Involvement of the alpha(1)-AR was confirmed by exposure of myocytes to the alpha(1)-AR agonist phenylephrine, which induced a similar profile of PKD autophosphorylation to norepinephrine (EC50 0.6 mu M). The effects of both alpha(1)-AR stimulation and PMA on PKD autophosphorylation were mediated by PKC, since these effects could be attenuated by pretreatment of myocytes with the PKC inhibitor bisindolylmaleimide. These data show that PKD is expressed in rat ventricular myocardium, where its expression is subject to developmental control, and that PKD activity in ventricular myocytes is regulated through alpha(1)-AR-and PKC-mediated pathways. (C) 2000 Academic Press.