Journal
ANALYTICA CHIMICA ACTA
Volume 874, Issue -, Pages 59-65Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2015.03.021
Keywords
Surface plasmon resonance; Signal amplification; MicroRNA; DNA super-sandwich assemblies; Streptavidin
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Funding
- Direct For Mathematical & Physical Scien [1413449] Funding Source: National Science Foundation
- Division Of Chemistry [1413449] Funding Source: National Science Foundation
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MicroRNAs (miRNAs) play an important regulatory role in cells and dysregulation of miRNA has been associated with a variety of diseases, making them a promising biomarker. In this work, a novel biosensing strategy has been developed for label-free detection of miRNA using surface plasmon resonance (SPR) coupled with DNA super-sandwich assemblies and biotin-strepavidin based amplification. The target miRNA is selectively captured by surface-bound DNA probes. After hybridization, streptavidin is employed for signal amplification via binding with biotin on the long DNA super-sandwich assemblies, resulting in a large increase of the SPR signal. The method shows very high sensitivity, capable of detecting miRNA at the concentration down to 9 pM with a wide dynamic range of 6 orders of magnitude (from 1 x 10 (11) M to 1 x 10 (6) M) in 30 min, and excellent specificity with discriminating a single base mismatched miRNA sequence. This biosensor exhibits good reproducibility and precision, and has been successfully applied to the detection of miRNA in total RNA samples extracted from human breast adenocarcinoma MCF-7 cells. It, therefore, offers a highly effective alternative approach for miRNA detection in biomedical research and clinical diagnosis. (C) 2015 Elsevier B.V. All rights reserved.
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