Journal
TROPICAL MEDICINE & INTERNATIONAL HEALTH
Volume 5, Issue 6, Pages 438-442Publisher
BLACKWELL SCIENCE LTD
DOI: 10.1046/j.1365-3156.2000.00565.x
Keywords
glutathione; L-buthionine sulphoximine (BSO); Leishmania donovani; nitric oxide
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This study focuses on the use of buthioinine sulphoximine (BSO), a gamma-glutamylcysteine synthetase inhibitor, oil Leishmania donovani growth. The effect of BSO on amastigote multiplication within macrophages showed that 5 mM BSO decreased infectivity by about 50% and the mean number of amastigotes per 100 infected macrophages by 21%. The mechanism may be that BSO resulted in enhanced nitric oxide (NO] levels within macrophages, probably due to inhibition of GSH content since GSH (10 mM) given after BSO treatment led to a decrease in NO compared to macrophages treated with BSO alone which were preexposed to thr Leishmania surface molecule lipophosphoglycan.
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