Extension of anther culture to several genotypes of cultivated oats

To improve plant regeneration from oat anther culture, the basic medium, hormonal supplements and genotype effect were studied, Sire of the 14 genotypes tested regenerated plants. Cultivars Kolbu, Katri. Stout and naked oat Lisbeth produced green plants, cultivars Virma and line OT 257 only albinos. The total number of green plantlets regenerated was 22, of which 13 (11 haploid, 2 doubled haploid) survived into the greenhouse, and 37 albinos. Regenerable-type embryos were induced from heat-pretreated anthers on media containing 2, 3 or 5 mg l(-1) 2,4-dichlorophenoxyacetic acid and 0.2 or 0.5 mg l(-1) kinetin as hormonal supplements. 6-Benzylaminopurine promoted albino plant regeneration especially in W-14 medium. Colchicine treatment was applied successfully to haploid regenerants.