Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 41, Issue 1, Pages 19-21Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0167-7012(00)00136-6
Keywords
dead cells; dye uptake; phloxin B
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A rapid and simple assay was developed for detection of yeast colonies containing dying or dead cells. Methylene blue, phloxin B, rose bengal and trypan blue at concentrations of 5-10 mu mol l(-1) were shown to stain non-viable cells in colonies of Saccharomyces cerevisiae, Schizosaccharomyces pombe, Candida albicans and Filobasidium capsuligenum without staining or affecting the viability of living cells of the colonies. (C) 2000 Elsevier Science B.V. All rights reserved.
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