Journal
FERTILITY AND STERILITY
Volume 73, Issue 6, Pages 1209-1218Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/S0015-0282(00)00495-7
Keywords
Robertsonian translocation; reciprocal translocation; mosaicism; PGD
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Objective: To review 35 cases of preimplantation genetic diagnosis (PGD) of translocations with several methods, including telomeric probes. Design: Retrospective study. Setting: Clinical IVF laboratory. Patient(s): Thirty-five couples with one partner carrying a chromosomal translocation. Intervention(s): PGD of translocation after polar-body or embryo biopsy. Main Outcome Measure(s): Pregnancy outcome. Result(s): Several trends were observed. First, PGD can achieve a statistically significant reduction in spontaneous abortion, from 95% to 13%. Second, the chances of achieving pregnancy are correlated with 50% or more of the embryos being chromosomally normal. Third, patients with robertsonian translocations produced fewer abnormal gametes and more pregnancies than did patients with reciprocal translocations. Fourth, a new fluorescence in situ hybridization protocol for PGD of translocations, which involves applying telomeric probes, has proved adequately reliable with a 6% average error rate. Conclusion(s): PGD of translocations achieves a statistically significant reduction in spontaneous abortion, both for polar-body and blastomere biopsy cases. Pregnancy outcome depended on the number of normal embryos available for transfer, with patients having <50% abnormal embryos achieving the most pregnancies. Because robertsonian translocations caused fewer abnormal embryos than reciprocal translocations, they also resulted in higher rates of implantation. ((C) 2000 by American Society for Reproductive Medicine.)
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