Journal
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
Volume 24, Issue 6, Pages 403-409Publisher
NATURE AMERICA INC
DOI: 10.1038/sj.jim.7000006
Keywords
phenol; soil; enzyme; extracellular
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Two microorganisms isolated from Amazonian forest soil samples and identified as Candida tropicalis and Alcaligenes faecalis were capable of degrading phenol (16 and 12 mM, respectively) at high salt concentrations (15% and 5.6%, respectively). Chromatographic and enzymatic studies revealed that each microorganism cleaved phenol at the ortho position with total phenol mineralization. C-14-phenol mineralization assays showed that both microorganisms assimilated about 30% of the total label. No phenol degradation metabolite (i.e., catechol, cis, cis-muconic acid) was detected in the intercellular medium. The presence of phenol hydroxylase (EC 1.14.13.7) and catechol 1,2-dioxygenase (EC 1.13.11.1) extracellular activity suggested that these microorganisms may secrete these enzymes into the extracellular medium.
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