Journal
SYSTEMATIC AND APPLIED MICROBIOLOGY
Volume 23, Issue 2, Pages 230-237Publisher
ELSEVIER GMBH
DOI: 10.1016/S0723-2020(00)80009-0
Keywords
enterococci; identification; phylogeny
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A PCR assay based on the use of degenerate oligodeoxyribonucleotides allowed characterization of a fragment internal to the dill genes encoding D-alanine:D-alanine ligases in Enterococcus columbae, E. durans, E. malodoratus, E. mundtii, E. raffinosus, E. seriolicida, E. solitarius, and E. sulfureus. Phylogenetic analysis of the sequence of the amplification products and of those already obtained from E. avium, E. casseliflavus, E. cecorum, E. dispar, E. faecalis, E. faecium, E. flavescens, E. gallinarum, E. hirae, E. pseudoavium, and E. saccharolyticus yielded an evolutionary tree with a topology similar to that based on 16S rRNA sequences. Partial sequencing of the ddl gene can therefore be used for genotypic identification of Enterococcus spp.
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