Journal
BIOSENSORS & BIOELECTRONICS
Volume 15, Issue 3-4, Pages 135-141Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/S0956-5663(00)00067-1
Keywords
acoustic wave device; Langmuir-Blodgett immobilization; antigen-antibody monolayers; sensor
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The rapid and sensitive detection of Salmonella typhymurium based on the use of a polyclonal antibody immobilized by the Langmuir-Blodgett method on the surface of a quartz crystal acoustic wave device was demonstrated. The binding of bacteria to the surface changed the crystal resonance parameters; these were quantified by the output voltage of the sensor instrumentation. The sensor had a lower detection limit of a few hundred cells/ml, and a response time of < 100 s over the range of 10(2)-10(10) cells/ml. The sensor response was linear between bacterial concentrations of 10(2)-10(7) cells/ml, with a sensitivity of 18 mV/decade. The binding of bacteria was specific with two binding sites needed to bind a single cell. The sensors preserve similar to 75% of their sensitivity over a period of 32 days. (C) 2000 Elsevier Science S.A. All rights reserved.
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