Journal
NATURE CELL BIOLOGY
Volume 2, Issue 6, Pages 313-317Publisher
MACMILLAN PUBLISHERS LTD
DOI: 10.1038/35014000
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Cell-cell adhesion mediated by specific cell-surface molecules is essential for multicellular development. Here we quantify de-adhesion forces at the resolution of individual cell-adhesion molecules, by controlling the interactions between single cells and combining single-molecule force spectroscopy with genetic manipulation. Our measurements are focused on a glycoprotein, contact site A (csA), as a prototype of cell-adhesion proteins, csA is expressed in aggregating cells of Dictyostelium discoideum, which are engaged in development of a multicellular organism. Adhesion between two adjacent cell surfaces involves discrete interactions characterized by an unbinding force of 23 +/-8 pN, measured at a rupture rate of 2.5+/-0.5 mu m s(-1).
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