Journal
FUNGAL GENETICS AND BIOLOGY
Volume 30, Issue 1, Pages 81-93Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1006/fgbi.2000.1205
Keywords
Xanthoria parietina and X. ectaneoides; hydrophobins; lichenized ascomycetes; rodlet layer; cell wall surface hydrophobicity
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In the symbiotic phenotype of the lichen-forming ascomycetes Xanthoria parietina and X. ectaneoides, a conglutinate, hydrophilic cortex surrounds a system of aerial hyphae with hydrophobic wall surfaces, In X, parietina freeze-fracture electron microscopy showed that a rodlet layer covers the fungal and algal wall surfaces. Extracts of hot SDS-insoluble wall residues isolated from both species contained a protein that revealed a rodlet layer upon interfacial self-assembly. The N-terminal sequence of the 10-kDa protein of X, ectaneoides served to clone cDNA fragments of XEH1 (H1 of X, ectaneoides) and XPH1 (H1 of X, parietina) by RT-PCR, Genomic DNA blot analysis with both lichenized species and the aposymbiotically cultured symbionts of X, parietina showed that XPH1 and XEH1 ave fungal single copy genes. The deduced amino acid sequences of the two encoded proteins were 96% identical and showed the characteristics of class hydrophobins. (C) 2000 Academic Press.
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