Journal
ANALYTICA CHIMICA ACTA
Volume 873, Issue -, Pages 88-98Publisher
ELSEVIER
DOI: 10.1016/j.aca.2015.03.001
Keywords
Fluorescence; Peptide; Biosensor; Chemosensor; Ratiometric; Heparin
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Funding
- Senior Research Program of the National Research Foundation [2014R1A2A1A11051727]
- Inha University Research Grant
- National Research Foundation of Korea [2014R1A2A1A11051727] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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New fluorescent peptide-based sensors (1-3) for monitoring heparin in serum sample were synthesized using short peptides (1 similar to 3mer) as a receptor. The peptide-based sensors (2 and 3) showed a sensitive ratiometric response to heparin both in aqueous buffered solution (10 mM HEPES, pH 7.4) and in 2% human serum sample by increase of excimer emission of pyrene at 480 nm and concomitant decrease of monomer emission of pyrene at 376 nm, whereas the peptide-based sensor 1 showed a turn off response only by decrease of monomer emission at 376 nm. 2 and 3 exhibited excellent selectivity toward heparin among various anions and competitors of heparin including chondroitin 4-sulfate (ChS) and hyaluronic acid (HA). Peptide-based sensor 3 showed a more sensitive response to heparin than 2. The detection limit of 3 was determined as 36 pM (R-2 = 0.998) for heparin in aqueous solution and 204 pM (R-2 = 0.999) for heparin in aqueous solutions containing 2% human serum. The peptide-based sensors, 2 and 3 provided a practical and potential tool for the detection and quantification of heparin in real biological samples. (C) 2015 Elsevier B.V. All rights reserved.
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