Journal
PHYTOCHEMISTRY
Volume 54, Issue 4, Pages 387-392Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0031-9422(00)00098-4
Keywords
Trifolium pratense; Leguminosae; red clover; hydroxycinnamic acid amide; clovamide; jasmonic acid; induction
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The effect of jasmonic acid (JA) on the secondary metabolism of 5-day-old red clover seedlings was investigated. Induction of the formation of four compounds was found in roots after treatment with 50 mu M JA for 48 h, while no induction was observed in the shoots. These compounds, whose formation was induced by JA addition, were isolated and identified as caffeoyl DOPA (clovamide), caffeoyltyrosine, p-coumaroyl DOPA and p-coumaroyltyrosine by ion-spray MS and H-1 NMR analyses, and by chemical synthesis. Among them, clovamide was the most abundant, while the other amides represented only a minor portion. Clovamide started to increase in amount 24-36 h after treatment and reached a maximum after 96 h (2.81 nmol/mg fr. wt.). The induction of their formation was observed even with 5 mu M of JA, and the amount increased with concentrations up to 100 mu M. Treatment with 1 mM CuCl2, which elicits accumulation of the phytoalexin maackiain in red clover, caused a decrease in clovamide amount. (C) 2000 Elsevier Science Ltd. All lights reserved.
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