Journal
JOURNAL OF VETERINARY MEDICAL SCIENCE
Volume 62, Issue 6, Pages 661-664Publisher
JAPAN SOC VET SCI
DOI: 10.1292/jvms.62.661
Keywords
bovine; ELISA; IL-1ra
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A sandwich ELISA for the bovine IL-1 receptor antagonist (bIL-1ra) was developed using recombinant (r) bIL-1ra produced by Escherichia coli, anti-rbIL-1ra rabbit IgG, its biotinylated one and avidin-peroxidase. This ELISA system enabled detection of rbIL-1ra at a concentration of more than 2 ng/ml. This ELISA was applied to quantitation of bIL-1ra in sera and whey of mastitic and healthy cows. The results indicate thar although IL-1ra levels in healthy and mastitic sera and whey were comparable, serum IL-1ra/IL-1P beta ratio of euthanized cows was significantly lower than that of the recovered.
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