Journal
FEBS LETTERS
Volume 474, Issue 2-3, Pages 137-140Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(00)01587-8
Keywords
lipid peroxidation; polymorphonuclear leukocyte; imaging; diphenyl-1-pyrenylphosphine; fluorescent probe
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Diphenyl-1-pyrrenylphosphine (DPPP) was tested whether it could be used as a fluorescent probe to monitor lipid peroxidation in cell membranes. DPPP reacted with organic hydroperoxides and hydrogen peroxide stoichiometrically to give DPPP oxide (DPPP = O), DPPP incorporated into phosphatidylcholine liposomal membranes and polymorphonuclear leukocytes (PMNs) reacted with methyl linoleate hydroperoxide rapidly but not with hydrogen peroxide nor wi:th tert-butyl hydroperoxide. This novel method revealed that lipid peroxidation proceeded within membranes of PMNs stimulated with phorbol 12-myristate 13-acetate, which is known to produce several, kinds of free radicals, It was found that DPPP is a suitable fluorescent probe to monitor lipid peroxidation within cell membranes specifically, (C) 2000 Federation of European Biochemical Societies.
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