Journal
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
Volume 1501, Issue 2-3, Pages 189-199Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0925-4439(00)00021-1
Keywords
scrapie strain 263K; transmissible spongiform encephalopathy; microspectroscopy; spectral mapping; cerebellum; medulla oblongata; pons
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In this report a new approach for the identification of pathological changes in scrapie-infected Syrian hamster brains using Fourier transform infrared microspectroscopy is discussed. Using computer-based pattern recognition techniques and imaging, infrared maps with high structural contrast were obtained. This strategy permitted comparison of spectroscopic data from identical anatomical structures in scrapie-infected and control brains. Consistent alterations in membrane state-of-order, protein composition, carbohydrate and nucleic acid constituents were detected in scrapie-infected tissues. Cluster analysis performed on spectra of homogenized medulla oblongata and pens samples also reliably separated uninfected from infected specimens. This method provides a useful tool not only for the exploration of the disease process but also for the development of rapid diagnostic and screening techniques of transmissible spongiform encephalopathies. (C) 2000 Elsevier Science B.V. All rights reserved.
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