Journal
FEBS LETTERS
Volume 475, Issue 3, Pages 213-217Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(00)01680-X
Keywords
protein phosphorylation; (plant) mitochondrion; inner membrane; matrix; protein kinase; protein phosphatase; protein kinase phosphatase
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Inside-out inner mitochondrial membranes free of matrix proteins mere isolated from purified potato tuber (Solanum tuberosum L,) mitochondria and incubated with [gamma-P-32]ATP, Proteins were separated by SDS-PAGE and visualized by autoradiography. Phosphorylation of inner membrane proteins, including ATPase subunits, was strongly inhibited by the phosphoprotein phosphatase inhibitor NaF. We propose that an inner membrane phosphoprotein phosphatase is required for activation of the inner membrane protein kinase, When prelabelled inner membranes were incubated in the absence of [gamma-P-32]ATP, there was no phosphoprotein dephosphorylation unless a soluble matrix fraction was added, This dephosphorylation was inhibited by NaF, but not by okadaic acid. We conclude that the mitochondrial matrix contains a phosphoprotein phosphatase that is responsible for dephosphorylation of inner membrane phosphoproteins. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V, All rights reserved.
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