Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 275, Issue 25, Pages 18864-18870Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M002810200
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- NIGMS NIH HHS [R01 GM049650, R01 GM056244, R37 GM049650, GM49650, GM56244] Funding Source: Medline
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ATP-dependent chromatin remodeling enzymes antagonize the inhibitory effects of chromatin. We com pare six different remodeling complexes: ySWI/SNF, yRSC, hSWI/SNF, xMi-2, dCHRAC, and dNURF. We find that each complex uses similar amounts of ATP to remodel nucleosomal arrays at nearly identical rates. We also perform assays with arrays reconstituted with hyperacetylated or trypsinized histones and isolated histone (H3/H4)(2) tetramers. The results define three groups of the ATP-dependent family of remodeling enzymes. In addition we investigate the ability of an acidic activator to recruit remodeling complexes to nucleosomal arrays. We propose that ATP-dependent chromatin remodeling enzymes share a common reaction mechanism and that a key distinction between complexes is in their mode of regulation or recruitment.
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