Journal
FEMS MICROBIOLOGY ECOLOGY
Volume 33, Issue 1, Pages 21-25Publisher
ELSEVIER SCIENCE BV
DOI: 10.1111/j.1574-6941.2000.tb00722.x
Keywords
biosensor; Lux operon; Pseudomonas aeruginosa; recA; SOS regulon; DNA-damaging chemical
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Pseudomonas aeruginosa strain RM4440 carries a plasmid-borne fusion of the P. aeruginosa recA gene promoter to a promoterless lux operon from Vibrio fisheri. We tested the response of RM4440 in a biofilm to exposure to a 1-h pulse of each of 17 chemicals known to be toxic to bacteria and other organisms. The induction of light produced from the recA-lux fusion present in RM4440 proved to be sensitive and specific for DNA-damaging chemicals when included in a biofilm environment. This study demonstrates the potential usefulness of this construct for in situ investigation of bacterial communities. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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