Journal
THEORETICAL AND APPLIED GENETICS
Volume 101, Issue 1-2, Pages 1-6Publisher
SPRINGER
DOI: 10.1007/s001220051441
Keywords
agricultural biotechnology; gene transfer; soybean; imazapyr
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Imazapyr is a herbicidal molecule that concentrates in the apical meristematic region of the plant. Its mechanism of action is the inhibition of the enzymatic activity of acetohydroxyacid synthase, which catalyses the initial step in the biosynthesis of isoleucine, leucine and valine. The selectable marker gene, ahas, was previously isolated from Ambidopsis thaliana and contains a mutation at position 653 bp. Combining the use of imazapyr, the ahas gene and a multiple shooting induction protocol has allowed us to develop a novel system to select transgenic meristematic cells after the physical introduction of foreign genes. In this study, we describe a protocol to obtain a high frequency of fertile transgenic soybean plants that is variety-independent.
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