Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 66, Issue 7, Pages 3113-3116Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.66.7.3113-3116.2000
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Environmental DNA libraries prepared from three different soil samples were screened for genes conferring lipolytic activity on Escherichia coli clones. Screening on triolein agar revealed 1 positive clone out of 730,000 clones, and screening on tributyrin agar revealed 3 positive clones out of 286,000 E. coli clones. Substrate specificity analysis revealed that one recombinant strain harbored a lipase and the other three contained esterases. The genes responsible for the lipolytic activity were identified and characterized.
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