Journal
ELECTROCHEMISTRY COMMUNICATIONS
Volume 2, Issue 7, Pages 457-460Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/S1388-2481(00)00060-6
Keywords
ion-channel biosensors; supported bilayer lipid membranes; Campylobacter species; electrochemical detection; planar lipid bilayers
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This paper presents a new ion-channel biosensor based on supported bilayer lipid membrane for direct and fast detection of Campylobacter species. The sensing element of a biosensor is composed of a stainless-steel working electrode, which is covered by artificial bilayer lipid membrane (BLM). Antibodies to bacteria embedded into the BLM are used as channel forming proteins. The biosensor has a strong signal amplification effect, which is defined as the total number of ions transported across the BLM. The total number of (univalent) ions flowing through the channels is 10(10) ions s(-1). The biosensor showed a very good sensitivity and selectivity to Campylobacter species. (C) 2000 Elsevier Science S.A. All rights reserved.
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