Journal
CYTOKINE
Volume 12, Issue 7, Pages 909-913Publisher
W B SAUNDERS CO
DOI: 10.1006/cyto.1999.0647
Keywords
gene expression; macrophage; MIF; particles; PCR
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This study analysed MIF mRNA and protein expression in human macrophages exposed in vitro to polymethylmethacryate and titanium alloy particles, MIF levels released from macrophages without exposure to particles mere in the range of 2-4 ng/ml, Exposure of macrophages to particles as demonstrated increased MIF release at 0.075%-0.225% v/v particle concentration, which was maximal at 12-24 h, MIF mRNA signal levels in cells with and without particles at a concentration of 0.075% showed no significant differences in a time course experiment. The profile of MIF release in response to increasing particle concentration coincided with increased release of lactate dehydrogenase, The viability of the cells was unchanged by the addition of particles as determined by 3H-thymidine uptake. These data suggest that MIF expression may represent an independent macrophage response to locally high particle concentrations. (C) 2000 Academic Press.
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