Journal
PLANT PHYSIOLOGY
Volume 123, Issue 3, Pages 795-805Publisher
OXFORD UNIV PRESS INC
DOI: 10.1104/pp.123.3.795
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Positional (or map-based) cloning techniques are widely used to identify the protein products of genes defined by mutation. In Arabidopsis the information generated by the Genome Initiative is giving this approach a decisive boost. A wealth of sequence polymorphisms and molecular markers is now available and can be exploited for fine mapping with technically simple and robust polymerase chain reaction-based methods. As a result it has become possible to complete positional cloning projects in a short time and with relatively little effort.
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