Journal
NEUROSCIENCE RESEARCH
Volume 37, Issue 3, Pages 183-190Publisher
ELSEVIER SCI IRELAND LTD
DOI: 10.1016/S0168-0102(00)00116-4
Keywords
nociceptor; visceral nociceptor; histamine; protein kinase C; sensitization; heat response
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To clarify the possible contribution of protein kinase C activation in histamine-induced excitation and sensitization of the heat response of testicular polymodal receptors, the effects of staurosporine, a protein kinase C inhibitor, and phorbol 12,13-dibutyrate, a protein kinase C activating phorbol ester, were studied in visceral polymodal receptors. Single polymodal receptor activities were recorded in vitro from testis-spermatic nerve preparations obtained from deeply anesthetized dogs (pentobarbital sodium, 30 mg/kg, i.v.). Histamine (10 mu M)-induced excitation and facilitation of the heat response of polymodal receptors were both suppressed by staurosporine (1 mu M), suggesting that activation of protein kinase C is involved in both these effects of histamine. Application of phorbol 12,13-dibutyrate (0.1 mu M) mixed with histamine increased the histamine-induced excitation, whereas a 5 min application of phorbol 12,13-dibutyrate before histamine suppressed it. These results suggest that phorbol 12,13-dibutyrate-activated protein kinase C has inactivation as well as activation effects on the intracellular cascade connected to histamine receptors, and that the former has a slower time course. (C) 2000 Elsevier Science Ireland Ltd and the Japan Neuroscience Society. All rights reserved.
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