Journal
BIOLOGICAL TRACE ELEMENT RESEARCH
Volume 152, Issue 1, Pages 135-142Publisher
HUMANA PRESS INC
DOI: 10.1007/s12011-013-9602-2
Keywords
Trace element; Vanadium ion; Osteoblast; Differentiation; Toxicity
Funding
- Department of Biotechnology, Government of India [DBT-JRF/08-09/459]
- Ministry of Human Resource and Development [MHR03-412]
- Uttarakhand State Council for Science and Technology [UCS&T/R&D/LS-10/11-12/4224/1]
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In recent time, vanadium compounds are being used as antidiabetic drug and in orthopedic implants. However, the exact role of this incorporated vanadium in improving the quality of bone structure and morphology is not known. The impact of vanadium ion was studied and compared to other trace metal ions with respect to the proliferation and osteoblast differentiation of C3H10t1/2 cells. Toxicity profile of these trace metal ions revealed a descending toxicity trend of Fe2+ > Zn2+ > Cu2+ > Co2+ > Mn2+ > V5+ > Cr2+. The effect of vanadium and other trace metal ions on osteoblast differentiation was evaluated by culturing the cells for 10 days in osteoblastic medium supplemented with different trace ions at concentrations lower than their cytotoxic doses. The results indicated that vanadium has maximum impact on the induction of osteoblast differentiation by upregulating alkaline phosphatase activity and mineralization by up to 145 and 150 %, respectively (p < 0.05), over control. Cu2+ and Zn2+ had a mild inhibitory effect, while Mn2+, Fe2+, and Co2+ demonstrated a clear decrease in osteoblast differentiation when compared to the control. The data as presented here demonstrate that orthopedic implants, if supplemented with trace metals like vanadium, may provide a source of better model for bone formation and its turnover.
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