Journal
JOURNAL OF COMBINATORIAL CHEMISTRY
Volume 2, Issue 4, Pages 355-360Publisher
AMER CHEMICAL SOC
DOI: 10.1021/cc0000139
Keywords
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Funding
- NCI NIH HHS [R21 CA84708] Funding Source: Medline
- NHGRI NIH HHS [R43 HG01891] Funding Source: Medline
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Solution reactions using photogenerated reagents (PGRs) (Gao, X.; Yu, P. Y.; Leproust, E.; Sonigo, L.; Pellois, J. P.; Zhang, H. J. Am. Chem. Sec. 1998, 120, 12698) are developed for parallel synthesis of addressable, combinatorial molecular microarrays. To advance the PGR chemistry for general chemical conversions, light-controlled synthesis of peptides, which employs photogenerated acids (PGAs) and/or in combination with photosensitizers for deprotection of N-t-Boc group, is demonstrated. These reactions were performed on resin and glass plates and conveniently monitored by HPLC analysis (reactions on resin) and fluorescence emission after coupling the deprotected NH2 group with 4(5)-carboxyfluorescein. These results demonstrate the potential of the PGA chemistry for parallel synthesis of addressable peptide libraries on a microarray platform.
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