Journal
BRAIN RESEARCH BULLETIN
Volume 52, Issue 4, Pages 297-302Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0361-9230(00)00265-3
Keywords
mu-opioid receptor knockout mice; G-protein coupling; mu-, delta-, and kappa-opioid receptor
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Mice lacking the mu-opioid receptor gene have been developed by a gene knockout procedure. In this study, the activity of opioid receptor coupled G-proteins was examined to investigate whether there is a change in the extent of coupling for mu-, delta-, and kappa-opioid receptors in mu-opioid receptor knockout mice. Selective agonists of mu- (DAMGO), delta- (DPDPE), and kappa-(U-69,593) opioid receptors stimulated [S-35]GTP gamma S binding in the caudate putamen and cortex of wild-type mice. In contrast, only U-69,593 stimulated [S-35]GTP gamma S binding in these regions of mu-opioid receptor knockout mice. These results confirmed the absence of G-protein activation by a mu-opioid receptor agonist in mu-opioid receptor knockout mice, and demonstrated that coupling of the kappa-opioid receptor to G-proteins is preserved in these mice. However, G-protein activation by the S-opioid receptor agonist, DPDPE, was reduced in the mu-opioid receptor knockout mice, at least in the brain regions studied using autoradiography. (C) 2000 Elsevier Science Inc.
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