Journal
JOURNAL OF VIROLOGY
Volume 74, Issue 13, Pages 6050-6057Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.74.13.6050-6057.2000
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Funding
- NHLBI NIH HHS [HL54734, P01 HL051818, HL51818] Funding Source: Medline
- NIAID NIH HHS [AI35667] Funding Source: Medline
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Lumenal delivery of adenovirus vectors (AdV) results in inefficient gene transfer to human airway epithelium, The human coxsackievirus and adenovirus receptor (hCAR) was detected by immunofluorescence selectively at the basolateral surfaces of freshly excised human airway epithelial cells, suggesting that the absence of epical hCAR constitutes a barrier to adenovirus-mediated gene delivery in vivo. In transfected polarized Madin-Darby canine kidney cells, wild-type hCAR was expressed selectively at the basolateral membrane, whereas hCAR lacking the transmembrane and/or cytoplasmic domains was expressed on both the basolateral and apical membranes. Cells expressing apical hCAR still were not efficiently transduced by AdV applied to the apical surface. However, after the cells were treated with agents that remove components of the apical surface glycocalyx, AdV transduction occurred. These results indicate that adenovirus can infect via receptors located at the apical cell membrane but that the glycocalyx impedes interaction of AdV with apical receptors.
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