Journal
BIOLOGICAL TRACE ELEMENT RESEARCH
Volume 145, Issue 1, Pages 1-9Publisher
SPRINGERNATURE
DOI: 10.1007/s12011-011-9154-2
Keywords
Sodium selenite; Osteosarcoma; ROS; Caspase; Apoptosis
Funding
- National Natural Science Foundation of China [90919050]
- Natural Science Foundation of Guangdong Province [8251063201000008]
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Sodium selenite (Na2SeO3, SSE) is an inorganic Se compound that is widely used in cancer chemoprevention studies. SSE has been shown to have anti-proliferative effects on several types of human cancer cells, but its effect on osteosarcoma cells has thus far not been reported. In this study, the cytotoxic effect of SSE on osteosarcoma cells U2OS was investigated in vitro and found to be higher than on comparable non-cancer cell lines 293 and L6. Treatment with SSE decreased cell growth in a dose- and time-dependent manner and altered cellular morphology. SSE also inhibited cell viability by inducing apoptosis, as evidenced by the formation of apoptotic bodies, generation of reactive oxygen species (ROS), and accumulation of cells during the advanced phase of apoptosis. SSE-induced apoptosis correlated with the activation of CASP 3, downregulation of BCL-2, and upregulation of P53 and PTEN in U2OS cells. These results indicated that SSE induces apoptosis in U2OS cells mainly through an ROS-mediated caspase pathway. This is the first report to show a possible mechanism of the anti-proliferative effect of SSE for the prevention of osteosarcoma in cell culture models.
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