Journal
NATURE BIOTECHNOLOGY
Volume 18, Issue 7, Pages 764-767Publisher
NATURE AMERICA INC
DOI: 10.1038/77337
Keywords
nanoprobe; nanotechnology; bioprobe; antibody; benzo[a]pyrene; benzopyrene tetrol; DNA damage; DNA adduct; carcinogen; intracellular measurement
Categories
Ask authors/readers for more resources
We report here the application of an antibody-based nanoprobe for in situ measurements of a single cell. The nanoprobe employs antibody-based receptors targeted to a fluorescent analyte, benzopyrene tetrol (BPT), a metabolite of the carcinogen benzo[a]pyrene (BaP) and of the BaP-DNA adduct. Detection of BPT is of great biomedical interest, since this species can serve as a biomarker for monitoring DNA damage due to BaP exposure and for possible precancer diagnosis. The measurements were performed on the rat liver epithelial clone 9 cell line, which was used as the model cell system. Before making measurements, the cells were treated with BPT. Nanoprobes were inserted into individual cells, incubated 5 min to allow antigen-antibody binding, and then removed for fluorescence detection. We determined a concentration of 9.6 +/- 0.2 x 10(-11) M for BPT in the individual cells investigated. The results demonstrate the possibility of in situ measurements inside a single cell using an antibody-based nanoprobe.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available