Journal
BIOLOGICAL CHEMISTRY
Volume 381, Issue 7, Pages 611-614Publisher
WALTER DE GRUYTER GMBH
DOI: 10.1515/BC.2000.078
Keywords
gene regulation; glycerol phosphate shuttle; mGPDH; primary hepatocytes; tissue-specificity
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The mitochondrial FAD-dependent glycerol-3-phosphate dehydrogenase (mGPDH) is an essential component of the glycerol phosphate shuttle which transfers reduction equivalents from the cytoplasm into the mitochondria. We analyzed the distribution of different exon 1-containing transcripts by RT-PCR in various tissues in vivo. Exon la was predominantly expressed in brain, brown adipose tissue and pancreas, exon Ib was ubiquitously expressed, and exon Ic was exclusively expressed in testis. In transient transfection assays the ubiquitous promoter B showed a detectable activity, whereas promoters A and C were completely silent. A deletion mutational analysis located the basal promoter B activity to a 316 bp core sequence upstream of the transcription start site.
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