Journal
CANADIAN JOURNAL OF PLANT SCIENCE
Volume 80, Issue 3, Pages 583-585Publisher
AGR INST CANADA
DOI: 10.4141/P99-118
Keywords
common buckwheat (F. esculentum Moench.); sperm cell isolation; osmotic shock
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Protoplasts from sperm cells were isolated. Sperm cells from germinated pollen placed in solution were released, sieved and removed after sucrose density gradient centrifugation. The isolated cells were spherical and approximately 5.0-6.0 mu m in diameter. Cytological observations confirmed that sperm cells of common buckwheat are true protoplasts.
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