Journal
GUT
Volume 47, Issue 1, Pages 18-22Publisher
BRITISH MED JOURNAL PUBL GROUP
DOI: 10.1136/gut.47.1.18
Keywords
Lewis blood group antigens; cagA; iceA; vacA; Helicobacter pylori; peptic ulcer
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Background-Studies in Western populations suggest that cagA, iceA, and vacA gene status in helicobacter pylori isolates is associated with increased virulence and peptic ulcer disease. Aim-To investigate the relationship between peptic ulcer and expression of Lewis (Le) antigens as well as cagA, iceA, and vacA in H pylori isolates in Singapore.;Methods-Expression of Le antigens in H pylori isolates obtained from patients with dyspepsia was measured by enzyme Linked immunosorbent assay. The cagA, iceA, and vacA status was determined by polymerase chain reaction. Results-Of 108 H pylori isolates, 103 (95.4%) expressed Le(x) and/or Le(y), while Le(a) and Le(b) were expressed in 23 (21.3%) and 47 (43.5%) isolates, respectively. Expression of two or more Le antigens (Le(x), Le(y), Le(a), or Le(b)) was significantly higher in H pylori isolated from ulcer patients than in non-ulcer patients (89.6% v 73.2%, p=0.035). There were no significant differences in the prevalence of cagA or iceA1 in H pylori isolates from peptic ulcer and non-ulcer patients (86.6% v 90.2% for cagA; 70.1% v 68.3% for iceA1), and no association of peptic ulcer with any specific vacA genotype. Conclusions-The present study indicates that peptic ulcer disease is associated with increased expression of Lewis antigens but not cagA, iceA, or vacA genotype in H pylori isolates in our population. This suggests that cagA, iceA, and vacA are not universal virulence markers, and that host-pathogen interactions are important in determining clinical outcome.
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