Journal
EMBO JOURNAL
Volume 19, Issue 14, Pages 3770-3777Publisher
OXFORD UNIV PRESS
DOI: 10.1093/emboj/19.14.3770
Keywords
cleavage; 3 ' end processing; polyadenylation; transcriptional termination
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Using a coupled, in vitro transcription and polyadenylation system we have investigated the molecular mechanism of transcriptional termination by RNA polymerase II (PolII), We showed previously that specific G-rich sequences pause transcription and then activate polyadenylation. We show that physiological pause sites activate polyadenylation in our in vitro system, We also investigate the mechanism of PolII transcriptional termination, and show that these transcripts are either directly released from the transcription complex or are 3' end processed while still attached to the complex. We also show that 3' product (generated by cleavage/polyadenylation) remains associated with the transcription complex, but is rapidly degraded on it.
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