Journal
ONCOGENE
Volume 19, Issue 31, Pages 3546-3555Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.onc.1203693
Keywords
type I collagen; transforming growth factor-beta; coactivators; p300/CBP; signal transduction; Smads
Funding
- NIAMS NIH HHS [AR-46390, AR-42309] Funding Source: Medline
Ask authors/readers for more resources
Transforming growth factor-beta (TGF-beta) stimulation of Type I collagen gene (COL1A2) transcription involves the Smad signal transduction pathway, but the mechanisms of Smad-mediated transcriptional activation are not fully understood. We now demonstrate that the ubiquitous transcriptional coactivators p300 and CREB-binding protein (CBP) enhanced basal as well as TGF-beta- or Smad3-induced COL1A2 promoter activity, and stimulated the expression of endogenous Type I collagen. The adenoviral E1A oncoprotein abrogated stimulation of COL1A2 activity in transfected fibroblasts, and reduced the basal level of collagen gene expression. This effect was due to specific interaction of E1A with cellular p300/CBP because (a) a mutant form of EIA defective in p300 binding failed to abrogate stimulation, and (b) forced expression of D300/CBP restored the ability of TGF-beta to stimulate COL1A2 promoter activity in the presence of EIA, The effect of p300 on COL1A2, transcription appeared to be due, in part, to its intrinsic acetyltransferase activity, as stimulation induced by a histone acetyltransferase-deficient mutant p300 was substantially reduced. Transactivation of COL1A2 by p300 involved the Smad signaling pathway, as Smad4-deficient cells failed to respond to p300, and stimulation nas rescued by overexpression of Smad4, Furthermore, minimal constructs containing only the Smad-binding CAGACA element of COL1A2 were transactivated by p300 in the presence of TGF-beta, These results indicate, for the first time, that the multifunctional p300/CBP coactivators play a major role in Smad-dependent TGF-beta stimulation of collagen gene expression in fibroblasts.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available