Journal
FEBS LETTERS
Volume 477, Issue 3, Pages 249-252Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(00)01746-4
Keywords
glycosaminoglycan; phospholipid; membrane; microdomain
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Glycosaminoglycan side chains of membrane proteoglycans have been claimed to be located at the outermost layer of the glycocalyx surrounding the cell. In this study measurements by surface plasmon resonance and solid-phase assay have shown that both chondroitin sulfate and keratan sulfate but not heparin associate with phosphatidylcholine under physiological conditions. Spectrophotometric measurements also showed that chondroitin sulfate restricts the lateral diffusion of phosphatidylcholine in liposomes, These findings indicate that chondroitin sulfate and/or keratan sulfate chains of membrane proteoglycans crouch on the surface of the membrane while heparan sulfate chains stretch outward from the membrane surface as postulated traditionally, (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V, All rights reserved.
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