Journal
FEBS LETTERS
Volume 478, Issue 1-2, Pages 113-118Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(00)01831-7
Keywords
epithelial cell; gallbladder; cytotoxicity; mucin secretion; oxysterol; cholesterol
Ask authors/readers for more resources
In order to investigate oxysterol-mediated effects on the biliary system, we studied the effects of cholestan-3 beta,5 alpha,6 beta-triol (TriolC) and 7-ketocholesterol (7KC) on gallbladder epithelial cells, We compared their cell proliferation effects in cultured dog gallbladder epithelial cells (DGBE) to their effects in cultured human pulmonary artery endothelial cells (HPAE). Oxysterols inhibited cell proliferation in a dose-dependent fashion. Oxysterols inhibited cell growth to 50% of control at a higher dose for DGBE cells than for HPAE cells. TriolC was more cytotoxic than 7KC. We also investigated the effect of oxysterols on bile salt-induced mucin secretion by DGBE cells, TriolC suppressed mucin secretion bgl DGBE cells, whereas 7KC did not. These findings support the hypothesis that biliary oxysterols affect gallbladder mucosal function. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V.,All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available