Journal
FEBS LETTERS
Volume 478, Issue 1-2, Pages 187-191Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(00)01848-2
Keywords
phospholipase A(2); secretory phospholipase A(2); group X secretory phospholipase A(2); phospholipase A(2) receptor; lipid mediator
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Phospholipase A(2) receptor (PLA(2)R) mediates various biological responses elicited by group IB secretory phospholipase A(2) (sPLA(2)-IB). The recently cloned group X sPLA(2) (sPLA(2)-X) possesses several structural features characteristic of sPLA(2)-IB. Here, we detected a specific binding site of sPLA(2)-X in mouse osteoblastic MC3T3-E-1 cells. Cross-linking experiments demonstrated its molecular weight (180 kDa) to be similar to that of PLA(2)R, In fact, sPLA(2)-X was found to bind the recombinant PLA(2)R expressed in COS-7 cells, and its specific binding detected in mouse lung membranes was abolished by the deficiency of PLA(2)R. These findings demonstrate sPLA(2)-X to be one of the high-affinity ligands for mouse PLA(2)R, (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
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