Journal
JOURNAL OF NEUROSCIENCE METHODS
Volume 100, Issue 1-2, Pages 33-39Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0165-0270(00)00227-2
Keywords
hippocampal dentate gyrus; mossy fiber terminals; ultrastructural location of zinc; silver plating; tungstate physical developer
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The Timm's sulfide silver method is Frequently used for the demonstration of the mossy fiber bundle or sprouted mossy fibers in the normal or epileptic hippocampal dentate gyrus. Under the light microscope the results are excellent, but the ultrastructure is considerably impaired;Ind the silver grains produced are too large as compared to the sizes of intra-synaptic structures. The present study was meant to test a series of physical developers containing, instead of gum arabic, sodium tungstate as protective colloid. One of them left the ultrastructure fairly intact and produced small, round silver grains, making it possible to precisely locate zinc in mossy terminals. With this method, it could be demonstrated that zinc is contained inside synaptic vesicles in the resting axon terminals of granule cells. As a consequence of prolonged sodium sulfide perfusion, zinc is released from synaptic vesicles and enters the synaptic cleft. (C) 2000 Elsevier Science B.V. All rights reserved.
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